Journal: Journal of Food and Drug Analysis
Article Title: Untargeted metabolomics analysis assisted by signal selection for comprehensively identifying metabolites of new psychoactive substances: 4-MeO-α-PVP as an example
doi: 10.38212/2224-6614.3447
Figure Lengend Snippet: Analytical approach. One NPS, 4-MeO-α-PVP, with different concentration were incubated with human liver S9 followed by LC-MS analysis. Features in the raw LC-MS files were identified by Progenesis QI software. Matched features were further submitted to statistical analysis by using a web tool, MetaboFinder. Differentially expressed features were filtered out and identified by targeted LC-MS/MS analysis.
Article Snippet: Pooled human liver S9 fraction (20 mg protein/mL, from 150 individual donors) was purchased from Corning ® UltraPoolTM (Woburn, MA, USA).
Techniques: Concentration Assay, Incubation, Liquid Chromatography with Mass Spectroscopy, Software